Melatonin CAS 73-31-4 Assay 98.5%-101.5% (HPLC) High Purity
Shanghai Ruifu Chemical Co., Ltd. is the leading manufacturer of Melatonin (CAS: 73-31-4) with high quality, production capacity 50 tons per year. Ruifu Chemical has been supplying Melatonin more than 15 years.
Ruifu Chemical can provide worldwide delivery, competitive price, excellent service.
Purchase Melatonin, please contact us by e-mail: alvin@ruifuchem.com
| Chemical Name | Melatonin |
| Synonyms | N-Acetyl-5-Methoxytryptamine; Regulin; MT |
| Stock Status | In Stock, Production Capacity 50 Tons/Year |
| CAS Number | 73-31-4 |
| Molecular Formula | C13H16N2O2 |
| Molecular Weight | 232.28 g/mol |
| Melting Point | 116.0 to 120.0℃ |
| Store Under Inert Gas | Store Under Inert Gas |
| Sensitive | Light Sensitive, Air Sensitive |
| Solubility | Soluble in Methanol, Almost Transparency |
| Storage Temperature | Store Long-Term at 2-8℃ |
| COA & MSDS | Available |
| Sample | Available |
| Origin of Product | Shanghai, China |
| Product Categories |
Pharmaceutical Intermediates |
| Shelf Life | 2 Years if Sealed and Stored Properly |
| Brand | Ruifu Chemical |
| Items | Specifications | Results |
| Appearance | White to Off-White Crystalline Powder | Complies |
| Melting Point | 116.0-120.0℃ | 117.3-117.8℃ |
| Water by Karl Fischer | ≤0.30% | 0.21% |
| Residue on Ignition | ≤0.10% | 0.08% |
| Chloride | ≤0.02% | <0.02% |
| Individual Impurity | ≤0.10% | <0.10% |
| Total Impurities | ≤1.00% | <1.00% |
| Heavy Metals (Pb) | ≤10ppm | <10ppm |
| Arsenic (as As) | ≤1.0ppm | <1.0ppm |
| Assay / Analysis Method | 98.5%-101.5% (HPLC) (Calculated on the Dried Basis) | 99.62% |
| Infrared Spectrum | Conforms to Structure | Conforms |
| Conclusion | The product has been tested and complies with the given specifications | |
Package: Bottle, Aluminium foil bag, 25kg/cardboard drum, or according to customer's requirement.
Storage Condition: Keep the container tightly closed. Store in a cool, dry (2-8℃) and well-ventilated warehouse away from incompatible substances. Keep away from sunshine; avoid fire and heat sources; avoid moisture.
Shipping: Deliver to worldwide by air, by FedEx / DHL Express. Provide fast and reliable delivery.
Melatonin
C13H16N2O2 232.28
N-Acetyl-5-methoxytryptamine;
N-(2-(5-Methoxy-1H-indol-3-yl)ethyl) acetamide [73-31-4].
DEFINITION
Melatonin contains NLT 98.5% and NMT 101.5% of melatonin (C13H16N2O2), calculated on the dried basis.
IDENTIFICATION
Change to read:
• A. SPECTROSCOPIC IDENTIFICATION TESTS <197>, Infrared Spectroscopy: 197K (CN 1-MAY-2020)
Change to read:
• B. SPECTROSCOPIC IDENTIFICATION TESTS <197>, Ultraviolet-Visible Spectroscopy: 197U (CN 1-MAY-2020)
Analytical wavelength: 277 nm
Sample solution: 10 µg/mL of Melatonin in isopropyl alcohol
Acceptance criteria: Meets the requirements. Absorptivities, calculated on the dried basis, do not differ by more than 3.0%.
• C. HPLC IDENTIFICATION TEST
Analysis: Proceed as directed in the Assay.
Acceptance criteria: The retention time of the major peak of the Sample solution corresponds to that of the Standard solution.
ASSAY
• PROCEDURE
Buffer: 0.5 g/L of monobasic potassium phosphate in water. Adjust with phosphoric acid to a pH of 3.5, and lter.
Mobile phase: Acetonitrile and Buffer (25:75)
System suitability solution: 0.1 mg/mL of USP Melatonin RS and 0.02 mg/mL USP Melatonin Related Compound A RS in Mobile
phase
Standard solution: 0.1 mg/mL of USP Melatonin RS in Mobile phase
Sample solution: 0.1 mg/mL of Melatonin in Mobile phase
Chromatographic system
(See Chromatography <621>, System Suitability.)
Mode: LC
Detector: UV 222 nm
Column: 4.6-mm × 15-cm; 5-µm packing L1
Flow rate: 1.0 mL/min
Injection size: 10 µL
System suitability
Samples: System suitability solution and Standard solution
[NOTE-The relative retention times for melatonin related compound A and melatonin are 0.4 and 1.0, respectively.]
Suitability requirements
Resolution: NLT 4 between melatonin and melatonin related compound A, System suitability solution
Relative standard deviation: NMT 2.0%, Standard solution
Analysis
Samples: Standard solution and Sample solution
Calculate the percentage of melatonin in the portion of Melatonin taken:
Result = (rU /rS ) × (CS /CU ) × 100
rU = peak response from the Sample solution
rS= peak response from the Standard solution
CS = concentration of USP Melatonin RS in the Standard solution (mg/mL)
CU = concentration of Melatonin in the Sample solution (mg/mL)
Acceptance criteria: 98.5-101.5% on the dried basis
IMPURITIES
• RESIDUE ON IGNITION <281>: NMT 0.1%
• CHLORIDE AND SULFATE, Chloride<221>
Standard: 0.10 mL of 0.020 N hydrochloric acid
Sample: 0.36 g of Melatonin
Acceptance criteria: NMT 0.02%
• RELATED COMPOUNDS
Solution A: Acetonitrile
Solution B: Use Buffer, prepared as directed in the Assay.
Mobile phase: See Table 1.
Table 1
Time (min) Solution A (%) Solution B (%)
0 25 75
7 25 75
15 80 20
18 25 75
25 25 75
Diluent: Mixture of Solution A and Solution B (25:75)
System suitability solution: 0.1 mg/mL of USP Melatonin RS and 0.02 mg/mL of USP Melatonin Related Compound A RS in Diluent
Standard solution: 5 µg/mL of USP Melatonin RS in Diluent
Sample solution: 1 mg/mL of Melatonin in Diluent
Chromatographic system
(See Chromatography <621>, System Suitability.)
Mode: LC
Detector: UV 222 nm
Column: 4.6-mm × 15-cm; 5-µm packing L1
Flow rate: 1.0 mL/min
Injection size: 10 µL
System suitability
Sample: System suitability solution
[NOTE-The relative retention times for melatonin related compound A and melatonin are 0.4 and 1.0, respectively.]
Suitability requirements
Resolution: NLT 4.0 between melatonin and melatonin related compound A
Relative standard deviation: NMT 2.0% for the melatonin peak
Analysis
Samples: Standard solution and Sample solution
Calculate the percentage of each individual impurity in the portion of Melatonin taken:
Result = (rU /rS ) × (CS /CU ) × 100
rU = peak response of each individual impurity from the Sample solution
rS = peak response of melatonin from the Standard solution
CS = concentration of USP Melatonin RS in the Standard solution (mg/mL)
CU = concentration of Melatonin in the Sample solution (mg/mL)
Acceptance criteria
Individual impurities: NMT 0.1%
Total impurities: NMT 1.0%
SPECIFIC TESTS
• LOSS ON DRYING <731>
Analysis: Dry a sample at 80° in a vacuum for 3 h.
Acceptance criteria: NMT 1.0%
ADDITIONAL REQUIREMENTS
• PACKAGING AND STORAGE: Preserve in tight containers, protected from light.
• USP REFERENCE STANDARDS <11>
USP Melatonin RS
USP Melatonin Related Compound A RS
2-(5-Methoxy-1H-indol-3-yl)ethanamine.
C11H14N2O 190.24
How to Purchase? Please contact Dr. Alvin Huang: sales@ruifuchem.com or alvin@ruifuchem.com
15 Years Experience? We have more than 15 years of experience in the manufacture and export of a wide range of high quality pharmaceutical intermediates or fine chemicals.
Main Markets? Sell to domestic market, North America, Europe, India, Korea, Japanese, Australia, etc.
Advantages? Superior quality, affordable price, professional services and technical support, fast delivery.
Quality Assurance? Strict quality control system. Professional equipment for analysis include NMR, LC-MS, GC, HPLC, ICP-MS, UV, IR, OR, K.F, ROI, LOD, MP, Clarity, Solubility, Microbial limit test, etc.
Samples? Most products provide free samples for quality evaluation, shipping cost should be paid by customers.
Factory Audit? Factory audit welcome. Please make an appointment in advance.
MOQ? No MOQ. Small order is acceptable.
Delivery Time? If within stock, three days delivery guaranteed.
Transportation? By Express (FedEx, DHL), by Air, by Sea.
Documents? After sales service: COA, MOA, ROS, MSDS, etc. can be provided.
Custom Synthesis? Can provide custom synthesis services to best fit your research needs.
Payment Terms? Proforma invoice will be sent first after confirmation of order, enclosed our bank information. Payment by T/T (Telex Transfer), PayPal, Western Union, etc.
Risk Codes
R60 - May impair fertility
R39/23/24/25 -
R23/24/25 - Toxic by inhalation, in contact with skin and if swallowed.
R11 - Highly Flammable
Safety Description
S24/25 - Avoid contact with skin and eyes.
S99 -
S53 - Avoid exposure - obtain special instructions before use.
S45 - In case of accident or if you feel unwell, seek medical advice immediately (show the label whenever possible.)
S36/37 - Wear suitable protective clothing and gloves.
S16 - Keep away from sources of ignition.
S7 - Keep container tightly closed.
UN IDs UN1230 - class 3 - PG 2 - Methanol, solution
WGK Germany 2
RTECS AC5955000
FLUKA BRAND F CODES 8-10-23
HS Code 2937900090
Toxicity LD50 orally in Rabbit: > 3200 mg/kg
Melatonin (CAS: 73-31-4) is a hormone produced by the pineal gland. Melatonin is is a hormone that regulates sleep-wake cycles. It is primarily released by the pineal gland. As a supplement, it is often used for the short-term treatment of trouble sleeping such as from jet lag or shift work. Melatonin can be widely used in health products, foods, pharmaceuticals, various cosmetics, nourishing care products.
1. Melatonin and freckle removal, skin whitening: Melatonin powder has the good skin whitening effect. melatonin can whiten the skin. It can regulate human endocrine, remove dark spots, age spots, chloasma, pregnancy spots and pigmentation caused by sun exposure, whiten, moisturize and restore youthful appearance to the skin.
2. Effectively improve sleep: Appropriate supplementation of Melatonin can effectively improve sleep quality.
3. Prolong life: Melatonin is the most important high-efficiency endogenous antioxidant in the brain, which has a protective effect on the brain. Therefore, melatonin is an important anti-aging hormone. Melatonin powder can improve human immunity, prevent depression, Alzheimer's syndrome and cataracts; 6).Melatonin powder can increase the body's immune system and enhance physical fitness.
4. Maintain and enhance sexual function: Supplementing melatonin can improve the sexual function of middle-aged and elderly people who are frail and energy-decreasing, resulting in loss of libido.
